Hayato M. Yamanouchi

Journal Articles

Back to all publications

  • Hayato M Yamanouchi*, Ryosuke F Takeuchi, Naoya Chiba, Koichi Hashimoto, Takashi Shimizu, Ryoya Tanaka*, Azusa Kamikouchi*

    YORU: social behavior detection based on user-defined animal appearance using deep learning

    bioRxiv · 2024

    DOI: 10.1101/2024.11.12.623320

    *Corresponding author

  • Gaurav Das, Olfat A. Malak, Khushboo Sharma, Abdalla G. Alia, Swetha Gopalakrishnan, Reshma V. Menon, Hayato M. Yamanouchi, Akinao Nose, Hokto Kazama, Adrian W. Moore, Takashi Suzuki

    The Fly Brain Lands in Tokyo: A Report on The 3rd Asia Pacific Drosophila Neurobiology Conference

    Genes to Cells · 2024

    DOI: 10.1111/gtc.13178

    A meeting report on APDNC3 (The 3rd Asia Pacific Drosophila Neurobiology Conference).

  • Hayato M Yamanouchi*, Azusa Kamikouchi, Ryoya Tanaka*

    Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system

    STAR Protocols · Volume 4, Issue 4 · 2023

    DOI: 10.1016/j.xpro.2023.102623

    *Corresponding author

    We report a new experimental method for optogenetically silencing neurons exclusively during copulation in Drosophila. This approach uses DeepLabCut-Live!, a machine learning-based tool for real-time pose estimation in animals, to detect copulation in real time and to control laser illumination based on that detection.

  • Hayato M Yamanouchi, Ryoya Tanaka*, Azusa Kamikouchi*

    Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males

    iScience · Volume 26, Issue 5 · 2023

    DOI: 10.1016/j.isci.2023.106617

    We found that Piezo, a mechanosensitive ion channel, is involved in postural control during copulation in male fruit flies. We further showed that destabilizing the male’s copulation posture by blocking Piezo can reduce reproductive performance. These results indicate that males actively maintain their posture during copulation through mechanosensation, and that doing so contributes to reproductive success. We expect this work to help reveal the full picture of the molecular and neural mechanisms that control animal copulation behavior.